Western blot analysis from lysates of human liver tissue, human skeletal muscle tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47932) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for TMEM219 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions.